SOUTHERN BLOT Meaning and
Definition
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Southern blot is a molecular biology technique used to detect specific DNA sequences in a sample. It was named after its inventor, Dr. Edwin Southern, and is predominantly employed to study gene presence, size, and abundance.
The procedure involves several key steps. Initially, the DNA sample is extracted and purified. It is then digested with specific restriction enzymes, which cleave the DNA into smaller fragments. These fragments are then separated by size using gel electrophoresis, where an electric current is passed through a gel matrix, causing the DNA fragments to migrate based on their size.
After electrophoresis, the DNA fragments are transferred or “blotted” onto a solid support, typically a membrane. This step involves capillary action or vacuum transfer techniques. The DNA fragments now immobilized on the membrane are then probed with a labeled DNA or RNA probe that is complementary to the target sequence of interest. The probe hybridizes specifically to the target DNA sequence and any excess probe is washed away.
Finally, the immobilized DNA fragments are visualized using autoradiography or other detection methods, depending on the label used. This allows for the identification and analysis of the target DNA sequence in the original sample.
Southern blotting is commonly employed for DNA fingerprinting, genetic testing, and the analysis of gene expression patterns. It has greatly contributed to our understanding of genetics and the identification of disease-causing mutations.
Common Misspellings for SOUTHERN BLOT
- aouthern blot
- zouthern blot
- xouthern blot
- douthern blot
- eouthern blot
- wouthern blot
- siuthern blot
- skuthern blot
- sluthern blot
- sputhern blot
- s0uthern blot
- s9uthern blot
- soythern blot
- sohthern blot
- sojthern blot
- soithern blot
- so8thern blot
- so7thern blot
- sourhern blot
- soufhern blot
Etymology of SOUTHERN BLOT
The term "Southern blot" is named after its inventor, Edwin M. Southern, who developed the technique in 1975. Southern blotting is a molecular biology technique used for detecting specific sequences of DNA in a DNA sample. The name was coined as a play on words, combining Southern's last name with the word "blotting", which refers to the transfer of DNA from a gel onto a solid support, typically a nitrocellulose membrane.
Similar spelling word for SOUTHERN BLOT
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